Sample Data
Select from list to retrieve from database
Sort by 
V-bar
Direct Entry   Standard   Compute
No Temp Correction  V

Enter Partial Specific Volume

Value
Temperature at which this value was measured if not at 20 °C

 °C
Save to XML file Load from XML file
Buffer Data
Select from list to retrieve from database
Sort by 
Density
  Direct Entry   Standard   Compute
No Temp Correction ρ
Viscosity
  Direct Entry   Standard   Compute
No Temp Correction η
pH
 
pH
Calculate Buffer Density
Density

Density Corrected for Temperature & Isotopes of Water

Calculate Buffer Viscosity
Viscosity

Viscosity Corrected for Temperature
Components

test
Buffer Components Concentration Units
Heavy Isotopes of water
% Volume
% Volume
% Volume
% Volume

Enter Density

Value
Temperature at which this value was measured if not at 20 °C

 °C

Enter Viscosity

Value
Temperature at which this value was measured if not at 20 °C

 °C
Save to XML file Load from XML file
Experiment Data
Select from list to retreive from database
Sort by 
Sed Equilibrium
Sed Velocity
Results
Save to XML file Load from XML file
Results
Select from list to retreive from database
Sort by 
Use Results from Method
 Vbar Method    Teller Method
Hydration Options







Assumed Shape
 Prolate ellipsoid
 Oblate ellipsoid
 Cylinder

Rstokes
--- cm
Results from Vbar Method Results from Teller Method
Ro: Rp:
Fo: Fp:
f/fo: f/fp:
Smax: Smax:
Dmax: Dmax:
Delta1 max: Delta1 max:
max a/b: max a/b:
2a max:          nm 2a max:          nm
2b max:          nm 2b max:          nm
Graph a/b vs Hydration Save to XML file Load from XML file
Name Moles

Total Amino Acids (monomer)
N = Make Oligomer

Edit Amino Acid Sequence


0 
Do you want all cysteines converted to disulfides?
NameMoles
Use Denaturant

 Clear 
Charge per Monomer
Isoelectric Point: 
Charge at pH 7.0: 
Titrable alpha Carboxyl
Titrable alpha Amino

  
Amino end pKa
Carboxy end pKa
Absorbance Properties in 6M guanidine Hydrochloride
[H. Edelhoch (1967) Biochemistry 6, 1948-1954]
Molar Extinction at 280 nm: 
Absorbance per mg/ml at 280 nm: 

Estimated Absorbance Properties of Folded Protein in Water
[C.N. Pace et al. (1995) Protein Science 4, 2411-2423]
Molar Extinction at 280 nm: 
Absorbance per mg/ml at 280 nm: 


Absorbance / Extinction Spectrum
Graph
Use Calculated Vbar Estimate (25 C)

VBar
Use Calculated Molecular Weight

Mr
Use Calculated Hydration Estimate

Hydration

Sedimentation Intrepretation Program
SEDNTERP Daemon Version:
database structure revision level:

NOTE
This application is still under development
and contains many unimplemented or
incomplete features. It is provided as a
demonstration of the current work and
may change significantly prior to it's first
full release.

Created by

Tucker Hurton, University of New Hampshire
Allan Wright, University of New Hampshire
Greg Deubler, University of New Hampshire
Bill Bashir, University of New Hampshire

Based on the Original Program Created by

David B. Hayes, Magdalen College
Tom Laue, University of New Hampshire
John Philo, Alliance Protien Laboratories
Copyright 1995 - 2011, The University of New Hampshire

Created with support from

Biomolecular Interactions Technology Center (BITC)